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One seminoma with MMR deficiency and MSI confirmed by PCR. IHC on large sections revealed protein loss for MLH1 (A) and PMS2 (B) in tumor cells, whereas adjacent stromal and inflammatory cells as well as cells of the residual seminiferous tubules were positive. MSH2 (C) and MSH6 (D) were retained in the tumor cells. Original magnifications 40×. MMR, mismatch repair; MSI, microsatellite instability; PCR, polymerase chain reaction; IHC, immunohistochemistry.
