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. 2021 Mar;9(6):484. doi: 10.21037/atm-21-567

Figure 4.

Figure 4

The anticancer role of circ-RANBP9. (A) qRT-PCR was used to measure the expression level of circ-RANBP9 in LC cells transfected with si-circ-RANBP9 si-NC, mock, or circ-RANBP9. (B) The CCK-8 assay demonstrated increased or decreased viability of circ-RANBP9 knockdown or overexpressed LC cells, respectively. (C) Proliferation was validated using the EDU assay. Scale bar: 50 µm. (D) Cell motility was measured by cell invasion or migration assays using a transwell chamber with or without matrigel in cells transfected with the indicated vectors. Cells were dyed by 0.1% crystal violet. Scale bar: 100 µm. (E) Cell motility was measured in LC cells transfected with si-RANBP9 or RANBP9 using the wound healing assay. Scale bar: 200 µm. (F) The effects of circ-RANBP9 on the epithelial to mesenchymal transition process was measured in LC cells transfected with circ-RANBP9 overexpressed vector using the western blot assay. Data indicate mean ± SD, n=3; **, P<0.01; ***, P<0.001.