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. 2021 Mar;9(6):479. doi: 10.21037/atm-21-503

Figure 4.

Figure 4

ERK1/2 activation improved mitochondrial function in senescent cardiomyocytes under hypoxia. (A,B) CaMEK transduction decreased mitochondrial ROS and PD98059 increased mitochondrial ROS in senescent cardiomyocytes under hypoxia, as detected by MitoSOX staining. Typical images of MitoSOX staining and quantified results were shown in shown in (A) and (B), respectively. (C,D) CaMEK transduction increased SOD activity (C) and ATP level (D), and PD98059 had the opposite effects in senescent cardiomyocytes under hypoxia. (E,F) CaMEK transduction increased mitochondrial membrane potential, while PD98059 decreased mitochondrial membrane potential in senescent cardiomyocytes under hypoxia. JC-1 staining was applied to detect mitochondrial membrane potential. Typical images were shown in (E), and quantified results were shown in (F). Data were presented as the mean ± SD (n=6). **P<0.01.