Figure 1.

Generation of macrophage‐specific RGS12-KO mice. (A) Organization of the wild-type RGS12 allele, the floxed RGS12 targeting construct, the LysM-Cre⁺ transgene, and the recombinant RGS12 allele. Exons are shown as black boxes and are numbered. Cre: Cre recombinase coding sequence; neo: neomycin resistance gene; and loxP: loxP sites. (B) Immunoblot analysis of bone marrow macrophage (BMM) lysates isolated from the long bone showing the absence of RGS12 protein in RGS12-cKO mice. (C) Quantitative analysis of the results in (B). Note that an immunoreactive band for RGS12 (150 kD) is detectable in LysM-Cre⁺ mice, whereas LysM-Cre⁺;RGS12^fl/fl mice almost completely lack the RGS12 protein. ***, P<0.001, n=5.