Figure 2.

Quantification of autophagosome formation

Top row images show raw data projections for a representative sample of (A) park^+/+ and (C) park^-/- PPL1 neurons expressing mCherry-Atg8a and TH-antibody staining (blue). Red puncta indicate lipidated mCherry-Atg8a. In the second row, red, blue and grey isosurfaces highlight mCherry-Atg8a puncta selected by the imaging software. We counted and compared the number of puncta located within TH-labeled region (bottom row) and did not detect an effect of the park-null mutation on autophagosome formation (B). Scale bar represents 10 μm. Data are represented as mean ± SEM. The effect of parkin loss of function was determined using a Mann-Whitney test. This figure has been modified from (Cackovic et al., 2018) and falls under terms of the Creative Commons Attribution License (CC BY).