Skip to main content
. 2021 Feb 26;296:100483. doi: 10.1016/j.jbc.2021.100483

Figure 1.

Figure 1

MiR-223-3p is upregulated in the calcified aortas.A, experimental scheme of CKD-induced medial calcification. B, representative Alizarin Red S and Von Kossa staining of aortas in sham and CKD groups 6 weeks after nephrectomy. C, aortic calcium levels after induction of medial calcification. The calcium content was normalized to the weight of the aorta (n = 6–7 per group). D, heat map of the ten most upregulated miRNAs in the aortas of CKD mice. Data points represent the log2 TPM (Transcripts Per Million) mapped values of the indicated genes. E, MiR-223-3p expression in the aortas of sham, CKD and aged ApoE knockout mice (n = 3 per group). F, expression of miR-21a-5p, miR-29a-3p, miR-29b-3p, miR-139-5p and miR-214-3p in the aortas of aged ApoE knockout mice (n = 3 per group). Data are expressed as the mean ± SD. ∗p < 0.05, ∗∗p < 0.01 by unpaired two-tailed Student's t-test.