MiR-223-3p KO promotes atherosclerotic calcification.A, plasma triglyceride and B, total cholesterol concentrations of WT, KO, aged ApoE KO and aged DKO mice (n = 6–7 per group). C, representative Alizarin Red S staining and HE staining in the brachiocephalic artery (BCA) of WT and KO groups. D and E, representative Alizarin Red S staining, HE staining (D) and quantification (E) in the BCA of aged ApoE KO and DKO mice (n = 6–7 per group). Data are expressed as the mean ± SD. ∗p <0.05 by unpaired two-tailed Student’s t-test.