Figure 1.

Sequence diversity and properties of mature quail and chicken CATH peptides synthesized for antibacterial activity testing. Amino acid sequences of mature peptides were aligned by MEGA6. “G” and “P” with pink highlight indicate glycine and proline residues necessary for the hinge structure. Yellow highlight indicates amino acid substitution that were predicted to have possibly damaging effects on protein function. Orange highlight indicates amino acid substitutions that were predicted to have no effects on protein function. Red, blue, and green bold letters indicate cationic amino acids (K and R), acidic amino acids (D and E), and aromatic amino acid (F, Y, and W), respectively. Length: number of amino acid residues. Net charge at pH7 calculated by PepCalc.com-Peptide property (https://pepcalc.com/calculator). Hydropathy index (Kyte and Doolittle, 1982). Abbreviations: CATH, cathelicidin; MW, molecular weight.