Table 2.
Effect of synthetic CATH-derived peptides on viability of the gram-negative bacterium Escherichia coli.
| Locus peptide (see Figure 1) | Peptide concentration (μmol) |
||||
|---|---|---|---|---|---|
| 0.1 | 0.5 | 1 | 10 | 100 | |
| CATH1 | |||||
| CjCATH1 (CcCATH1) | 48.0 ± 7.8 | 32.8 ± 7.8 | 8.6 ± 1.5 | 2.9 ± 1.1 | 0 |
| GgCATH1 | 54.4 ± 9.4 | 27.4 ± 9.9 | 13.8 ± 2.1 | 5.4 ± 1.6 | 0 |
| CATH2 | |||||
| CjCATH2∗01∗03 | 66.9 ± 2.7a | 17.4 ± 5.6a | 6.4 ± 1.5a | 4.7 ± 0.7 | 0 |
| CjCATH2∗02 | 76.7 ± 4.3a | 18.7 ± 3.5a | 6.1 ± 0.7a | 4.8 ± 1.0 | 0 |
| CjCATH2∗04 (CcCATH2) | 79.3 ± 6.6a | 24.6 ± 4.8a | 10.5 ± 2.0a | 6.2 ± 1.8 | 0 |
| GgCATH2 | 97.8 ± 5.4b | 82.9 ± 1.8b | 34.7 ± 7.1b | 11.0 ± 4.4 | 0 |
| CATH3 | |||||
| CjCATH3 (GgCATH3) | 42.9 ± 1.7 | 22.4 ± 7.4 | 13.1 ± 0.5 | 6.3 ± 0.9 | 0 |
| CATHB1 | |||||
| CjCATHB1∗01∗02∗03 | 92.7 ± 6.9 | 43.0 ± 0.7a | 31.4 ± 3.0a | 14.2 ± 2.2 | 0 |
| CjCATHB1∗04 | 94.4 ± 0.5 | 69.9 ± 10.9b | 31.6 ± 4.3a | 12.3 ± 3.1 | 0 |
| GgCATHB1 | 97.5 ± 4.8 | 83.6 ± 3.1b | 62.6 ± 9.1b | 8.2 ± 1.1 | 0 |
a,bMeans followed by the same small letter in the same row are not significantly different (P < 0.05).
Results are presented as means ± SEM (n = 3). Concentration-dependent differences in each CATH: a > b.
Abbreviation: CATH, cathelicidin.