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. 2021 Mar 17;21:262–273. doi: 10.1016/j.omtm.2021.03.008

Figure 1.

Figure 1

Generation of Rh-iPSCs from Rh PBMCs

(A) Schematic illustration showing the reprogramming of PBMCs to Rh-iPSCs. (B) Phase contrast images of iPSC colonies from three individuals. Scale bars, 200 μm. (C) Summary of the Rh-iPSC generation. (D) RT-PCR analysis of SeV vectors. GAPDH was examined as an internal control. (E) RT-PCR analysis of pluripotency-associated genes. GAPDH was examined as an internal control. (F) Flow cytometric analysis of SSEA-4 expression. (G) Teratoma formation assay shows derivatives of all three germ layers. GE, gut-like epithelium; C, cartilage; M, muscle tissue; NC, neural crest; ML, melanocytes. Scale bars, 50 μm. (H) Chromosomal analysis of Rh-iPSCs.