Figure 3.

Differentiation of Rh-iPSCs into macrophages

(A) Schematic illustration of the differentiation into macrophages from HPCs. Phase contrast images of macrophage differentiation on days 24 and 34 (left and middle, respectively) and cytospins on day 34 (right). Scale bars, 50 μm. (B) Flow cytometric analysis of the macrophage phenotypes 34 days after the differentiation. (C) Macrophages were incubated with Alexa Fluor 594 Escherichia coli and observed 1 h later. Microscopic images show bioparticles localized in the macrophages. Scale bars, 100 μm. (D) Cytokine production by macrophages differentiated from Rh-iPSCs. Data are plotted as the mean ± SD of triplicate samples. ∗∗∗∗p < 0.0001. (E) Detection of SIVmac316 in iPSC-derived macrophages by ELISA. HSC-F is a cynomolgus monkey T cell line and control. Replication was monitored by determining the amount of p27 in the culture supernatant at days 1, 4, and 7 after the incubation. Data are plotted as the mean ± SD of triplicate samples. ∗p < 0.05, ∗∗∗∗p < 0.0001 for comparisons between SIV mac 316 and SIV mac 239. iMac, iPSC-derived macrophage.