Figure 5.
Deletion of TRIM5α enables HIV-1 virus infection
(A) Significant increase of HIV luminescence of TRIM5αKO iMacs compared to parental iMacs. Macrophages (1 × 105 cells/well) were infected with NL43-Luci/VSV-G (10 ng of p24 virus) in a 24-well plate. Luciferase activity was measured 2, 3, and 4 days after the infection. Data are plotted as the mean ± SD of triplicate samples and presented as three independent measurements. ∗p < 0.05, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 for comparisons between TRIM5αKO iMacs and parental iMacs. (B) NL43-Luci/VSV-G infection was inhibited by nevirapine (NVP). Macrophages (5 × 104 cells/well) were infected with NL43-Luci/VSV-G (6.6 ng of p24 virus) in a 96-well plate. Luciferase activity was measured 2 days after the infection. Data are plotted as the mean ± SD of triplicate samples and presented as three independent measurements of a single experiment. ∗∗∗∗p < 0.0001 for comparisons between TRIM5αKO iMacs and parental iMacs.