Table 2.

Results of off-target sequence analysis of top five off-target candidates determined by the CFD score of TRIM5 gRNA

	Position	Sequence (5′→3′)	No. of mismatches	CFD off-target score	KO iPSC #1	KO iPSC #2	KO iPSC #3
TRIM5 on-target site	chr14:67658119–110054350	CTACGACAAAACCAACGTCT CGG	–	1.0000	−14 bp, −14 bp	−5 bp, −14 bp	−14 bp, −14 bp
Potential off-target sites	chr4:103904324–103904346	CTATAATAAAACCAACATCT TGG	4	0.525778	WT	WT	WT
	chr2:76073316–76073338	CTAAGGCAAAAACAACATCT AGG	4	0.401003	WT	WT	WT
	chr2:98763243–98763265	CTGCCACAAACCCAACATCT TGG	4	0.179259	WT	WT	WT
	chr7:42594298–42594320	ATAAGATAAAACCAACGTCT GAG	3	0.177388	WT	WT	WT
	chr9:104127964–104127986	CTACAAAGAAACCAACTTCT AGG	4	0.119167	WT	WT	WT

CFD, cutting frequency determination; WT, wild-type.