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. 2021 Mar 13;24:403–415. doi: 10.1016/j.omtn.2021.03.005

Figure 1.

Figure 1

Repair outcomes of SpCas9-dual gRNA-induced deletions at 8 endogenous loci of 3 cell lines

(A) List of wild-type and NHBEJ alleles for 8 targeting loci. (B) PCR results of 8 loci in 3 cell lines edited with SpCas9 and the corresponding dual gRNAs (amplified from pool of triplicate transfections). (C) Sanger sequencing results of deletion alleles, indicated with white arrows in (B), of 2 representative loci (P1 and P8) from 3 human cell lines. Full sequencing results are shown in Figure S1.