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Quantification of NHBEJ with ICE
(A) Schematic illustration of NHBEJ. For ICE analysis, a NHBEJ control is generated by Topo cloning of the PCR product from the NHBEJ deletion allele. The pseudo gRNA spacer is the 20 nt sequence flanking the junction site. (B) Representative ICE analysis results of the P1 and P8 loci. Full ICE results are shown in Figure S2. (C) NHBEJ efficiency for the 8 endogenous loci in 3 cell lines.
