Table 2.
GCMS and LCMS parameters.
| Gas Chromatography (GC)-Mass Spectrometry (MS) Parameters (Myo-Inositol and NAA) |
Liquid Chromatography (LC)- Mass Spectrometry (MS) Parameters (Choline and Creatine) |
||
|---|---|---|---|
| GAS chromatography (GC) | Liquid chromatography (LC) | ||
| GC Column Injection Temp Constant Gas Flow Injection Mode Split Ratio Injection Volume Run Time |
J&W DB-5MSCapillary 30 m × 250 μm × 0.25 μm 280 °C 1 ml/min Split 10:1 1 μL 13.3 min |
LC Column LC Column Temp Injection Tray Temp Injection Volume Flow Rate Run Time |
Agilent Zorbax Eclipse C18 Rapid Resolution HD 100 × 2.1 mm 1.8 μ 40 °C 10 °C 5 μL 200 μL 6 min |
| GC oven Temp Program temperature 100 °C/min and hold time 1.0 min, oven ramp: 30 °C/min, temperature 320 °C/min, hold time; 5 min, run time; 13.3 min |
|||
| Retention Times Myo-Inositol N-Acetylaspartic Acid Myo-Inositol-D6 Malic Acid-D3 |
7.2 min 5.7 min 7.2 min 4.6 min |
Retention Times Choline Creatine Acetylcholine-D4 |
1.2 min 1.3 min 1.3 min |
| Mass spectometry (MS) | Mass spectometry (MS) | ||
|---|---|---|---|
| Source Temperature Quad Temperature Acquisition time |
230 °C 150 °C 5–13.3 min |
Ionisation Polarity Spray Voltage Capillary Temp Auxiliary Gas Flow Sheath Gas Flow Acquisition time Scan type: Scan Width: Scan Time Data type Numbers of Microscans Collision Gas Pressure: Divert Valve: |
Electrospray (ESI) Positive 3500 V 350 °C 0 Arb 50 Arb 6 min SRM 0.200 m/z 0.150 s Profile 1 1.5 mTorr into Waste: 0–0.3 min, into MS: 3–6 min |