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. 2021 Mar 13;11(11):5491–5510. doi: 10.7150/thno.55041

Figure 1.

Figure 1

Increases in miR-143/145 levels during estrogen-deficient osteoporosis and the attenuation of BMSCs function. (A) The EVs miRNA database showed the expression of miR-143/145 in different sources. (B) Pearson correlation indicated the ELISA-detected levels of estrogen in 90 serum samples. (C) miRNA in the serum collected from 90 females were purified by miRNeasy Serum/Plasma Spike-In Control kit and analyzed for miR-143/145 detection by Pearson correlation. miR-143/145 expression were normalized to the spike-in control. (D) The Pearson correlation coefficient was analyzed for salivary miR-143/145 in the paired females. (E) The paired BMD value was calculated by CBCT to estimate the bone state. (F) Comparison of miR-143 and miR-145 in Pre- and Post-BMSCs. (G) The protein expression of NANOG, SOX2, OCT4 and SATB2 were detected in Pre- and Post-groups, which derived from 3 independent individuals, respectively. (H) The expression of miR-143 and miR-145 in estrogen or ICI treated BMSCs by qRT-PCR. (I) BMSCs stimulated with estrogen or ICI were analyzed for core TFs and SATB2 markers by Western blot. (J) Calcified nodules in estrogen or ICI stimulated BMSCs. Results are presented as the mean ± S.D. *p < 0.05; **p < 0.01; #p > 0.05 by Student's t test and one-way ANOVA.