Figure 2.

In vitro cellular studies. (A) Effect of different types of HMCNs on suppression of H₂O₂-induced intracellular ROS production in TM cells. TM cells were incubated with test HMCNs for 24 h and further exposure to H₂O₂ for 24 h; cells incubated with no materials served as Control. Scale bars: 100 µm. (B) Intracellular levels of ROS were measured by the fluorescence intensity of DCFH-DA, with a microplate reader. (C) Levels of IL-6 released from TM cultures after incubation with test samples for 3 days. Unstimulated and TGF-β-stimulated cells without contacting the test HMCNs were the negative control (NC) and positive control (PC). Values are mean ± SD (n = 6). ^*P < 0.05 vs NC, CNP20 nm and CNP40 nm groups; ^#P <0.05 vs PC and CNP10 nm groups. (D) TEER values of rabbit corneal endothelial cells exposed to different types of HMCNs for 24 h. Control: without materials. Values are mean ± SD (n = 6). ^*P < 0.05 vs all groups.