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. 2021 Mar 20;11(11):5605–5619. doi: 10.7150/thno.55878

Figure 5.

Figure 5

Inhibition of HDAC1/2 attenuated TNFα-induced VCAM-1 expression in primary EC isolated from mouse aorta. (A) Schematic illustration of CpG islands in mouse Gata promoter. (B) EC were isolated from aorta of Apoe-/- mice. After 80-90% confluency, they were pretreated with Romi, and stimulated with murine TNFα (10 ng/mL) followed by Western blot analysis (n = 3). Increased Ac-H3 level confirmed Romi activity. (C-E) VCAM-1 (C), GATA6 (D) and Ac-STAT3 was quantified. (F-G) Mouse primary EC were incubated with Romi for 1 h followed by 4 h treatment with 10 ng/mL TNFα. A chromatin immunoprecipitation assay was applied to examine enrichment of STAT3 onto -1479/-1470 site of Gata6 promoter (F) and GATA6 onto -271/-255 site of Vcam1 promoter (G) (n = 4).