Figure 1.

Inhibition of CBD in mice PASMCs' hyperproliferation without cytotoxicity. A, Purity of mice PASMCs assessed by immunohistochemistry with the α-SMA antibody. Scale bar = 100 µm B, mRNA levels of Il6 in mice PASMCs, incubation with different concentrations of CBD and/or CoCl₂ at 200 µM for stimulating the hypoxia condition, n = 6 per group. C and D, Level of LDH assessed with LDH detection assay in both extracellular (death rate) and intracellular (cell viability) (n = 6 per group). E, Quantitative assessment of BrdU antibody to calculate the ratio of PASMCs proliferation. F, Immunofluorescence of BrdU positive ratio of the PASMCs (n = 7 per group), the nuclei of cells were stained with 4',6-diamidino-2-phenylindole (DAPI). The results were analyzed by one-way ANOVA followed by Bonferroni's multiple comparison test, *P < 0.05, **P < 0.01, ***P < 0.001 vs. the control group, and ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. the CoCl₂ group or mice PAH-PASMCs.