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. 2021 Mar 11;11(11):5267–5278. doi: 10.7150/thno.55571

Figure 4.

Figure 4

CBD improved mitochondrial function in mice PAH-PASMCs. A, Representative images of PASMCs isolated from the control and PAH mice labeled with MitoTracker after the treatment with CBD (10 µM) or the control vehicle for 2 h. Nuclei were counterstained using Hoechst 33342, scale bar = 10 µm. B and C, Quantification of mitochondrial proportion of lineage that longer than 1 µm (40 pixels, about 1 µm) and mean length of mitochondria in control PASMCs and PAH-PASMCs labeled with Mitotracker after treatment with or without CBD for 2 h, n = 20 per group. D, Immunoblotting for DRP1, FIS1, MFN2 proteins in mice PAH-PASMCs with CBD (10 µM) or vehicle for 12 h. All blots were re-probed for β-actin as control. The results were analyzed by one-way ANOVA followed by Bonferroni's multiple comparison test, *P < 0.05, **P < 0.01, ***P < 0.001 vs. the control group, and #P < 0.05, ##P < 0.01, ###P < 0.001 vs. the mice PAH-PASMCs.