Figure 2.

Ocular delivery efficiency of EXO_KV11. (A) FITC-labeled KV11 or EXO_KV11 was injected into CD-1 mice retro-orbitally. Fluorescence of the eyes was imaged at 6 and 12 h after the indicated injection. Ctrl represents the uninjected group. (B) Quantification of fluorescence intensity of eyes in (A) (n = 11 - 12 mice/group). (C) Ocular distribution of KV11 and EXO_KV11 in cryosections of retinas from CD-1 mice treated as in (A). For checking in vivo distribution, FITC-labeled peptides were used. Arrowheads indicate co-localization of ECs (red, IsoB4) and peptide (green, FITC). (D) Quantification of fluorescence intensity of FITC-peptide in IsoB4⁺ area in cryosections of retinas in (C) (n = 3 mice/group; at least 3 images per mouse were analyzed and the values were averaged. The pictures for analysis were taken from the whole retinas, including peripheral part and retinas next to the optic nerve). The data represent as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, Mann-Whitney test in (B), student's t test (two-tailed) in (D). Scale bars, 20 µm.