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. 2021 Mar 13;11(11):5525–5538. doi: 10.7150/thno.57510

Figure 1.

Figure 1

11A4-ABD shows high binding affinity and is internalized in HER2 expressing cells, regardless of albumin presence. A) Schematic representation of the different nanobody constructs. B) Binding to HER2 expressing BT-474 cells of IRDye800 conjugated 11A4-ABD (11A4-ABD-IR) alone (green) or in the presense of human serum albumin (HSA; red) or mouse serum albumin (MSA; blue). C) Competition binding of 11A4-ABD-IR to BT-474 cells in the presence of BALB/c mouse serum. D) Ratio of internalized over bound signal of 11A4-IR (yellow squares) or 11A4-ABD-IR (green circles) in the absence (open symbols, dashed lines) or presense of HSA (filled symboles, continuous lines) over time on BT-474 cells. The data shown are representative experiments. Values were plotted as mean ±SD (n = 3).