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. 2021 Mar 12;11(11):5387–5403. doi: 10.7150/thno.42345

Figure 4.

Figure 4

Identification of LCN2 as a target gene of PRMT1. (A) RNA-seq was performed to analyze gene expression changes in PRMT1 deleted or DCPT1061 treated Caki-1 cells. (B-C) The volcano plots were performed to show the significant differential expression genes in different groups. (D-E) Down-regulated genes were detected by RT-qPCR in PRMT1 deleted or DCPT1061 treated ccRCC cells. (F-G) LCN2 expression levels were detected by Western blot in PRMT1-deleted or DCPT1061 treated Caki-1 cells. (H-I) H4R3me2a binding at the three sections of LCN2 promoter in PRMT1-deleted or DCPT1061 treated Caki-1 cells. *P < 0.05, **P < 0.01, and ***P < 0.001.