Fig. 1. CD34⁺ cell recovery and viability, CD45⁺ cell viability and potency assay assessment of HPC grafts of both RD and URD after thawing and washing.

Single-platform flow cytometry-based analysis was used for viable CD34⁺ and CD45⁺ cells enumeration and measurement of viability, while a 14-day culture and automated cell counting were performed to report CFU counts and clonogenicity. CD34⁺ cell recovery was calculated as the ratio of absolute count of post-wash viable CD34⁺ cells to pre-cryopreservation absolute counts of viable CD34⁺ cells. Clonogenicity was calculated as the percentage of CFU count (×10⁴) to post-wash viable CD34⁺ cells absolute count (×10⁶). Data are presented as median ± interquartile ranges. Recovery, viabilities and clonogenicity are reported as percentages using the left y-axis. CFU is reported as count (×10⁴/kg) using the right y-axis. Open symbols represent RD, closed symbols represent URD. p values are reported above the groups using Mann–Whitney test for null hypothesis testing.