Figure 7.

Effects on neuron dendritic morphology. (a) Compound 20 and Pyr3 protect against Aβ-induced damage to dendrites. Mature hippocampal neurons (DIV = 14) were treated with CHO control medium, or naturally secreted Aβ-containing conditioned medium (7PA2)²⁵ or in combination with compound 20 or Pyr3 at different concentrations, for 16 h, followed by immunocytochemistry using an antibody against MAP2 (green) and DAPI (blue). Scale bar: 100 μm. (b) Lack of toxicity of the inhibitor compounds in neuronal culture. Hippocampal neurons (DIV = 14) were treated with compound 20 or Pyr3 alone at different concentrations, followed by immunocytochemistry staining with MAP2 (green) and DAPI (blue). Scale bar: 100 μm. (c) Quantification of dendritic length. Bars are mean ± SEM. **p < 0.01 (one-way ANOVA). ****p < 0.0001 (one-way ANOVA). n = 50 dendrites.