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. 2021 Apr 6;16:2633–2646. doi: 10.2147/IJN.S297571

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© 2021 Zhou et al.

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In vitro SDT effect and the reeducation of macrophages. Cytotoxicity of these nanoparticles in normoxic (A) and hypoxic (B) MCF-7 cells after exposed with US, *p < 0.05 versus MON@Ce6 groups (n = 6). (C) Intracellular reactive oxygen species (ROS) fluorescence images (bars =10 µm). (D) FACS quantitative analysis of ROS, *p < 0.05 versus HA-MON@Ce6 groups (normoxia), ^#p < 0.05 versus MON@Ce6 (hypoxia) groups (n = 6). (E) TNFα, CD86, CD206 and IL10 gene expression of cells after various treatments. *p < 0.05 versus PBS groups; ^#p < 0.05 versus free HA group (n = 6). (F) The secretion of H₂O₂ by macrophages after various treatments. *p < 0.05 versus PBS groups; ^#p < 0.05 versus free HA group. All the data represent the mean ± SD (n = 6).