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. 2021 Mar 19;24:462–476. doi: 10.1016/j.omtn.2021.03.013

Figure 7.

Figure 7

Cellular localization of siRNAs mediated by 599 and its peptide variants

(A) Confocal fluorescence microscopy analysis of CAL 27 cancer cells 2 h post-treatment with DY547-siCIP2A (red) in complex with 599 or its peptide variants at 50:1 Peptide:siRNA molar (12.5 N/P) ratios. Filopodia (green) were stained with the selective F-actin label Alexa Fluor 488 phalloidin and nuclei (blue) were counterstained with DAPI. Scale bar: 17 μm. (B) Confocal fluorescence microscopy images of 599 and RD3AD-mediated localization of DY547-siCIP2A in CAL 27 cancer cells from (A) separated into their individual fluorophore signals. The merged images are also presented. Scale bar: 17 μm. (C) Confocal fluorescence microscopy analysis of SCC-90 cancer cells 2 h post-treatment with DY547-siCIP2A (red) in complex with 599 or RD3AD at a 50:1 Peptide:siRNA molar (12.5 N/P) ratio. Filopodia (green) were stained with the selective F-actin label Alexa Fluor 488 phalloidin and nuclei (blue) were counterstained with DAPI. Scale bar: 27 μm.