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. 2021 Feb 26;296:100477. doi: 10.1016/j.jbc.2021.100477

Figure 4.

Figure 4

Detection of candidate Siglec-7 ligand molecules by specific antibodies. K562 lysates (20 μg) and Siglec-7 ligand fractions purified from the lysates were treated with (+) or without (−) with sialidase (Sphingobacterium multivorum sialidase 12 mU, pH 7.4) and western blotting analyses were performed. The probes used were Siglec-7EcFc (Sig7EcFc) (A), anti-leukosialin (anti-SPN) (B), anti-nucleolin (anti-NCL) (C), and anti-heterogeneous nuclear ribonucleooprotein U (anti-HNRNPU) (D). Sialidase-derived staining is indicated by asterisks. The major sialidase sensitive specific bands are indicated by arrowheads.