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. 2021 Feb 26;296:100477. doi: 10.1016/j.jbc.2021.100477

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Immunoprecipitation of candidate molecules for Siglec-7 by specific antibodies. Leukosialin and nucleolin were immunoprecipitated with antileukosialin or antinucleolin antibodies (2 μl) from K562 lysate (200 μg). Controls were prepared without antibodies (control) via Protein A-Sepharose. Supernatant (sup) and precipitated proteins (bound) were denatured and analyzed by SDS-PAGE and western blotting. A, Siglec-7EcFc. B, anti-leukosialin. C, anti-nucleolin. D, anti-HNRNPU. Antibody and Protein A-derived staining are indicated by asterisks.