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. 2021 Mar 2;17(4):1026–1040. doi: 10.7150/ijbs.55559

Figure 2.

Figure 2

Production of Knock-in cell colony. A. Gene-targeting strategy of Cas9-mediated knock-in of VEGF or DsRed2 to the FGF5 locus. B. Gene names, types, and target sequences of the top 10 potential off-target sites of sgRNA were used in this study. C. Process of exploring the optimal HDR efficiency of small molecules. D. Spanning the homologous arms PCR identification results of cell colonies. E. Images of cell colonies at 3, 7, and 15 days. Scale bar = 100 µm. F. The sequences and locations of target sites at exon 1 of FGF5 gene.