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. 2021 Mar 2;17(4):995–1009. doi: 10.7150/ijbs.44907

Figure 1.

Figure 1

HHT inhibited breast cancer cells growth and proliferation. a. Effects of different concentrations of HHT on breast cancer cells, including MDA-MB-231, MCF-7,T47D and HCC1937 cells, while screening drug-sensitive cell lines. b. MDA-MB-231 cell was treated with different concentrations of HHT for 24 h, 48 h and 72 h. c. MCF-7 cell was treated with different concentrations of HHT for 24 h, 48 h and 72 h. Cellular proliferation inhibition rate was evaluated using CCK-8 assays, and the data are presented as the means ± SD. d. Images of MDA-MB-231 and MCF-7 cells positive for BrdU staining after treatment with indicated concentration of HHT, Scale bar =100 µm. The histogram demonstrates the results of BrdU-positive rate in MDA-MB-231 and MCF-7 cells. e. Colony formation assays were used to investigate the colony formation abilities of MDA-MB-231 and MCF-7 cells after treatment with the indicated concentrations of HHT. All data were analysed using unpaired Student's t-tests and are shown as the means ± SD. *p< 0.05, **p< 0.01.