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. 2021 Mar 1;17(4):957–971. doi: 10.7150/ijbs.52591

Figure 6.

Figure 6

PRSS1 affects cell proliferation via the PAR-2-activated ERK signaling pathway. (A) The expression of PAR2 protein was analyzed in GC tissues by immunohistochemistry analysis. (B) PAR-2 was highly expressed in MGC803 GC cells compared to GES-1 cells. (C) The effect of PRSS1 knockdown on PAR-2 protein expression in MGC803 cells. (D-E) The effect of FSLLRY-NH2 (PAR-2 inhibitor) and SLIGKV-NH2 (PAR-2 agonist) on the growth and proliferation of MGC803 GC cells was determined by MTT and colony formation assays. (F) Western blotting was performed to detect the expression levels of phosphorylated ERK1/2, PRSS1 and PAR2 in MGC803/miR-PRSS1 cells, MGC803 cells treated with FSLLRY-NH2 (PAR-2 inhibitor), and MGC803/miR-PRSS1 cells treated with SLIGKV-NH2 (PAR-2 agonist). Data are shown as the means ± SD, *P<0.05, **P<0.01, ***P<0.001; #P<0.05, ##P<0.01; ΔP<0.05.