Figure 4.

miR-128-3p inhibited SIRT1 expression by directly binding its 3'-UTR. (A) Schematic diagram of miR-128 targeting mutant and wild type seed sequences in the 3'-UTR of SIRT1. (B) Luciferase reporters harbouring putative target sites in the wild type and mutant 3'-UTRs of SIRT1 were cotransfected with 100 nM miR-128 mimic in HK-2 cells. qRT-PCR (C) and Western blot (D, E) were performed to detect the expression of SIRT1 in HK-2 cells transfected with the miR-128 mimic or inhibitor. β-actin served as an internal control. The data are shown as the mean ± SD of three independent experiments.