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. 2021 Mar 2;17(4):1050–1060. doi: 10.7150/ijbs.57160

Figure 5.

Figure 5

miR-128-3p partially reversed the effect of theaflavin on CaOx nephrocalcinosis-induced oxidative stress injury and crystal deposition in vivo. (A) CaOx crystal deposition in in theaflavin- and/or Agomir-128-treated mice was detected by polarized light optical microscope (100× magnification; scale bar: 50 µm) and Pizzolato staining (200× magnification; scale bar: 50 µm). (B) PAS staining illustrated tubular injury (200× magnification; scale bar: 20 µm). TUNEL staining detected renal tubular epithelial cell death (200× magnification; scale bar: 100 µm). (C) IHC was used to analyze NOX2, SOD2, and SIRT1 expression, and FISH to detect miR-128 expression in renal tissues (200×; scale bar: 100 µm). (D) Quantification of CaOx crystal deposition, PAS staining, TUNEL staining, NOX2, SOD2, and SIRT1 IHC staining, and miR-128 FISH. n = 6 per group. The data are shown as the mean ± SD. #p < 0.05 vs. the normal control group, *p < 0.05 vs. the glyoxylate group, $p < 0.05 vs. the Gly + TF 100 mg/kg group.