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. 2021 Mar 11;17(4):1138–1152. doi: 10.7150/ijbs.57825

Figure 3.

Figure 3

BA enhances autophagy after SCI. (A) Immunofluorescence staining for p62 and NeuN co-localization at the spinal cord lesion after SCI (scale bar = 25 µm). (B) The quantitative mean optical density of the p62 in motor neurons of spinal cord lesion in each group. (C) Immunofluorescence staining for LC3 and NeuN co-localization at the spinal cord lesion after SCI (scale bar = 25 µm). (D)The percentage of the LC3II positive neurons in motor neurons of spinal cord lesion in each group. (E) Western blotting for p62, LC3II, Beclin1, Vps34, and CTSD expression levels in the Sham, SCI and BA groups. The gels were run under the same experimental conditions, and the cropped blots are shown here. (F) The optical density values of the p62, LC3II, Beclin1, Vps34, and CTSD expression levels were quantified and analyzed in each group. The values are expressed as the means ± SEM, n=5 per group. **p< 0.01, vs. Sham group. ##p< 0.01, vs. SCI group.