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. 2021 Mar 11;17(4):1138–1152. doi: 10.7150/ijbs.57825

Figure 6.

Figure 6

BA attenuates mitophagy and reduces ROS accumulation after SCI. (A) ELISA of 8-OHdG, AOPP, and MDA in spinal cord lesions from Sham, SCI, BA and BA+3MA groups as indicated. (B) Immunofluorescence staining for Nix and NeuN co-localization in the spinal cords of the Sham, SCI, BA and BA+3MA groups (scale bar = 25 µm). (C) The quantitative mean optical density of the Nix in motor neurons of spinal cord lesion in each group. (D) Western blotting for Bnip3, Nix and Parkin expression levels in the Sham, SCI and BA groups. The gels were run under the same experimental conditions, and the cropped blots are shown here. (E) The optical density values of the Bnip3, Nix and Parkin expression levels were quantified and analyzed in the three groups. (F) Western blotting for Bnip3, Nix and Parkin expression levels in the BA and BA+3MA groups. The gels were run under the same experimental conditions, and the cropped blots are shown here. (G) The optical density values of the Bnip3, Nix and Parkin expression levels were quantified and analyzed in the both groups. The values are expressed as the means ± SEM, n=5 per group. *p< 0.05 and **p< 0.01, vs. Sham group. #p< 0.05 and ##p< 0.01, vs. SCI group. &p< 0.05 and &&p< 0.01, vs. BA group.