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. 2021 Mar 11;17(4):1138–1152. doi: 10.7150/ijbs.57825

Figure 7.

Figure 7

BA activates autophagy and mitophagy via enhancing AMPK-mTOR -TFEB activity. (A) Western blotting for AMPK, p-AMPK, mTOR and p-mTOR expression levels, and TFEB nuclear translocation in the Sham, SCI and BA groups. The gels were run under the same experimental conditions, and the cropped blots are shown here. (B) The optical density values of the AMPK, p-AMPK, mTOR, p-mTOR, normalized to the loading control GAPDH; Densitometric analysis of TFEB, normalized to the loading control H3. (C) Western blotting for AMPK, p-AMPK, mTOR and p-mTOR expression levels, and TFEB nuclear translocation in the BA and BA+3MA groups. The gels were run under the same experimental conditions, and the cropped blots are shown here. (D) The optical density values of the AMPK, p-AMPK, mTOR, p-mTOR, normalized to the loading control GAPDH; Densitometric analysis of TFEB, normalized to the loading control H3. (E) Western blotting for Caspase-1, NLRP3, GSDMD, p62, LC3II, Bnip3, Nix and Parkin expression levels in the BA and BA+CC groups. The gels were run under the same experimental conditions, and the cropped blots are shown here. (F) The optical density values of the Caspase-1, NLRP3, GSDMD, p62, LC3II, Bnip3, Nix and Parkin expression levels, normalized to the loading control GAPDH. The values are expressed as the means ± SEM, n=5 per group. *p< 0.05 and **p< 0.01, vs. Sham group. #p< 0.05 and ##p< 0.01, vs. SCI group. &p< 0.05 and &&p< 0.01, vs. BA group.