Figure 6.

Regulation of miR-223 expression in vivo and in vitro during HSC activation. (A) C57BL/6J mice were injected with CCl₄ for 4 weeks or 8 weeks. Hepatic miR-223 was measured by RT-qPCR. (B) C57BL/6J mice were injected with CCl₄ for 4 weeks. Primary HSCs were isolated from CCl₄-treated mice and control mice. Acta2, Col1a1, miR-223 and pri-223 expression levels were measured by RT-qPCR. (C) Primary HSCs were isolated and cultured for 1 day, 3 days, 5 days and 7 days, the expression of Acta2, Col1a1, miR-223 and pri-miR-223 was analyzed by RT-qPCR at indicated time points. Values represent means ± SEM. In panel A, n=5-8/group. Panels B-C from three independent experiments. *P< 0.05, **P< 0.01, ***P< 0.001.