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. Author manuscript; available in PMC: 2021 Apr 27.
Published in final edited form as: Sci Signal. 2020 Oct 27;13(655):eaay9217. doi: 10.1126/scisignal.aay9217

Fig. 4: TEPP-46 impacts enzymatic activity of PKM2, but not that of PKM1, but does not increase overall glycolytic flux in T cells.

Fig. 4:

(A) Pyruvate kinase (PK) activity assay on unstimulated CD4+ T cells from PKM2-WT and PKM2-KO mice treated with DMSO or TEPP-46 (50μM). Data are pooled from N=2–3 independent experiments with T cells freshly isolated from n=8 PKM2-WT and n=5 PKM2-KO mice. Significance was determined by repeated measures two-way ANOVA with Sidak post-test. (B and C) Assessment of WT CD4+ T cell functional metabolism by glycolytic (data pooled from N=3 independent experiments on CD4+ T cells from n=6 WT mice) (B) and mitochondrial stress tests (data pooled from N=2 independent experiments with n=4 WT mice) after 45 minutes of treatment with DMSO, TEPP-46 (50μM) or 2-deoxyglucose (2-DG, 1mM) (C). Significance determined by repeated measures two-way ANOVA with Bonferroni post-test. ****p<0.0001. All data are expressed as mean ± SEM. Oligo = oligomycin; FCCP = mitochondrial uncoupler; Rot/AA = rotenone + antimycin A.