Fig. 6: TEPP-46 enhances STAT5 activation during TH17 differentiation.
(A) Schematic of P-STAT3 and P-STAT5 effects on IL-17A and GM-CSF expression. (B) TH17 cells incubated with TEPP-46 (50μM) or vehicle (DMSO) for 24 hours were subjected to immunoblot analysis for phosphorylated STAT5 (P-STAT5) or total STAT5 (T-STAT5). Quantification is of n=7 mice pooled from N=3 independent experiments. Paired Student’s t-test. (C) Purified CD4+ T cells were skewed towards TH17 in the presence of TEPP-46 (50μM), ± STAT5 inhibitor (STAT5-IN-1, 100μM) for 48 hours, and subjected to flow cytometry analysis of GM-CSF and IL-17A expression. Quantification is of n=7 mice per group pooled from N=3 independent experiments. Repeated measures two-way ANOVA with Tukey post test. Significance is as follows: **p<0.01, ****p<0.0001. Data are presented as mean ± SEM.