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. 2021 Mar 11;18(9):2051–2062. doi: 10.7150/ijms.51497

Figure 3.

Figure 3

TIPE2 overexpression suppresses the viability and proliferation of PDAC cells. Real-time quantitative PCR (a-c) and western blot (d-g) analyses indicated that TIPE2 expression was markedly upregulated after transfection of the PRK5-TIPE2 plasmid in PANC-1 and SUIT-2 cells using PBMCs as a positive control. h-j. After TIPE2 overexpression, colony formation (h-i) and CCK8 (j) assays were conducted to evaluate the viability and proliferation of PANC-1 and SUIT-2 cells. Data represent the mean ± SD of three independent experiments. *P<0.05; **P<0.01; ***P<0.001.