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. 2021 Mar 3;18(9):1921–1934. doi: 10.7150/ijms.54052

Figure 3.

Figure 3

A-B. Quantification of the number of positive cells for the immunodetection of Osteocalcin (OCN) by Immunohistochemistry technics per field in the different study groups. C-H. Representative images of the protein expression of OCN in the middle layer (arrow: immunoprecipitation). NR<50: patients without reflux younger than 50 years, NR≥50: patients without reflux with 50 years or older, R<50: patients with reflux younger than 50 years, R≥50: patients with reflux with 50 years or older. I, intimal layer; M, middle layer; A, adventitial layer. * p <0.05.