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. 2021 Mar 3;18(9):1921–1934. doi: 10.7150/ijms.54052

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A-B. Intensity of Osteopontin (OPN) protein expression in venous wall in the different study groups. C-F. Images of protein expression by Osteopontin immunohistochemistry (OPN) by Immunohistochemistry technics in patients NR <50 (A), NR> 50 (B-C), R <50 (D-E) and R> 50 (F). Immunoprecipitation is brown. NR<50: patients without reflux younger than 50 years, NR≥50: patients without reflux with 50 years or older, R<50: patients with reflux younger than 50 years, R≥50: patients with reflux with 50 years or older. I, intimal layer; M, middle layer; A, adventitial layer. *p<0.05, ***p<0,001.