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. 2021 Mar 3;18(9):1921–1934. doi: 10.7150/ijms.54052

Figure 5.

Figure 5

Panel. A. Percentage distributions of patients showing positive protein expression for Pigment Epithelium‐Derived Factor (PEDF) by immunohistochemistry technics in patients without venous reflux (NR) and with reflux (R) stratified by age, n=number of patients. Panel. B. Images show the protein expression of PEDF through specific immunohistochemical detection in the patient groups NR<50 (A), NR≥50 (B), R<50 (C, D) and R≥50 (E). The specific precipitate correlated with the expression of this protein in the different vein layers appears in red. NR<50: patients without reflux younger than 50 years, NR≥50: patients without reflux with 50 years or older, R<50: patients with reflux younger than 50 years, R≥50: patients with reflux with 50 years or older. I, intimal layer; M, middle layer; A, adventitial layer.