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. 2021 Mar 19;18(10):2176–2186. doi: 10.7150/ijms.56564

Figure 3.

Figure 3

Effects 5-aza on UCP2, DNMT expression level and DNMT enzymatic activity in HT-29 cells. HT-29 cells were cultured for 24, 48, 72 h with 5-aza (0, 5, 10 μM). (A) The expression level of UCP2, DNMT1 and DNMT3a were analyzed by Western blot. The data are expressed as the mean±SEM of five independent experiments. *P < 0.05, **P< 0.01, ***P < 0.001 vs control group. (B) Nuclear proteins of HT-29 cells were isolated. The total nuclear protein concentration was normalized and DNMT activity assessed. The horizontal dotted line indicate baseline DNMT enzymatic activity of untreated control HT-29 cells group. The data are expressed as the mean±SEM of five independent experiments. *P < 0.05 vs control group.