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. 2021 Mar 19;18(10):2176–2186. doi: 10.7150/ijms.56564

Figure 4.

Figure 4

Effects 5-aza on UCP2, DNMT expression level and DNMT enzymatic activity in HepG2 cells. HepG2 cells were cultured for 24, 48, 72 h with 5-aza (0, 5, 10 μM). (A) The expression level of UCP2, DNMT1 and DNMT3a were analyzed by Western blot. The data are expressed as the mean±SEM of five independent experiments. ***P < 0.001 vs control group. (B) Nuclear proteins of HepG2 cells were isolated. The total nuclear protein concentration was normalized and DNMT activity assessed. The horizontal dotted line indicate baseline DNMT enzymatic activity of untreated control HepG2 cells group. The data are expressed as the mean±SEM of five independent experiments. *P < 0.05 vs control group.