Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primer sequences and PCR conditions for MSP analysis
| Gene Name | Primer sequence (5'-3') | Product size (bp) | Annealing temperature | ||
|---|---|---|---|---|---|
| Forward | Reverse | ||||
| UCP2 | U | GGATGGGTTAGTTAATTAAAGGT | ACCAAAACACTAAAAACCCCAA | 188 | 55℃ |
| M | ACGGGTTAGTTAGTTAATTAAAGGC | CCAAAACACTAAAAACCCCG | 191 | 55℃ | |
PCR was carried out in 43 cylces. (U) a specific unmethylated primer sequence, (M) a specific methylated primer sequence.
