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. 2021 Mar 15;17(5):1178–1190. doi: 10.7150/ijbs.57783

Figure 2.

Figure 2

Knockdown of circMETTL3 inhibits breast cancer cell proliferation in vitro and in vivo. A-B. qRT-PCR and western blot analysis of the transfection efficacy of siRNA on circMETTL3 and METTL3 expression in ZR-75-1 (A) and SUM1315 (B) cell lines after 48 h transfection. C. The proliferation ability of ZR-75-1 and SUM1315 cells lines transfected with siRNAs was determined by CCK-8 assays. D. The colony formation results of ZR-75-1 and SUM1315 cells lines transfected with siRNAs. Colonies > 50 mm were counted. E. EdU assays results of ZR-75-1 and SUM1315 cells lines transfected with siRNAs. Scale bar, 100 µm. Blue indicates DAPI, red indicates EdU, si-NC indicates negative control, si-1 indicates si-circMETTL3-1,si-2 indicates si-circMETTL3-2. F. Representative images of xenograft tumors of circMETTL3 overexpression (circMETTL3) and control (Vector) group (n = 6). G. Tumor weights in these two groups were shown. H. Tumor volumes were measured weekly in all mice that received subcutaneous injections. Data were shown as mean ± SD. *p <0.05, **p <0.01, ***p<0.001.