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. 2021 Mar 15;17(5):1178–1190. doi: 10.7150/ijbs.57783

Figure 4.

Figure 4

circMETTL3 acts as the sponge of miR-31-5p in breast cancer. A. FISH revealed the subcellular localization of circMETTL3. Red indicates circMETTL3, bule indicates DAPI. B. The Venn-diagram showed the potential miRNAs sponged by circMETTL3 predicted by combination of three bioinformatics algorithms (CircInteractome, circBank, starBase). C. Kaplan-Meier survival analysis showed the correlation between the expression of miR-31-5p and the overall survival of breast cancer patients based on TCGA. D. Knockdown of circMETTL3 increased expression of miR-31-5p in breast cancer cell lines. E. Correlation between circMETTL3 and METTL3 expression in breast cancer tissues. F. Schematic representation of circMETTL3-WT and circMETTL3-Mut luciferase reporter vectors (left). Luciferase reporter assay indicated the binding capacity of circMETTL3 with miR-31-5p (right). G. Anti-AGO2 RIP experiments were performed to detect circMETTL3 expression in breast cancer cell lines. ZR-75-1 and SUM1315 cells lysates were immunoprecipitated with AGO2 or IgG antibody and analyzed by using qRT-PCR to detect circMETTL3 transcript levels. H. RNA pull-down was performed to detect the enrichment of miR-31-5p in breast cancer cell lines. Data were shown as mean ± SD, *<0.05, **p <0.01, ***p<0.001.